Ads related to Microbiology and specifically Genomics and Metagenomics.
New positions, calls for grants, fellowships etc. Any brief announcement
that can be of interest to the community of genome and metagenome
If you are interested in publishing on this space, please contact
Professor Francisco Rodríguez Valera at firstname.lastname@example.org
- [07/12/2020] Follow-up to Daniel Portik's seminar
What is the best method for DNA fragmentation?
We recommend the Diagenode Megaruptor with Covaris g-Tubes. Recommendations for metagenomics samples can be found in the shotgun sequencing protocol.
Please note there is a section specific to the usage of the Covaris g-Tubes. The protocol is available here.
Is it possible to sequence full-length 16S and ITS?
Shoreline Biome has just released a new product that targets full-length 16S-ITS-23S amplicons, which are designed to be sequenced on PacBio Sequel II systems.
This is part of their StrainID product line. They recently presented a webinar on this topic:
Availability of PacBio metagenomics datasets:
HiFi data are publicly available for three libraries of a complex mock community dataset. This is ZymoBIOMICS gut microbiome standard D6331, a community containing 21
species that mimic the human gut microbiome. It includes staggered abundances across species (from 14% down to 0.0001%), as well as 5 strains of E. coli, making it a challenging community for methods testing.
The NCBI project page is: http://www.ncbi.nlm.nih.gov/bioproject/680590
Standard Input: SRX9569057
Low Input: SRX9569058
Ultra-Low Input: SRX9569059
PacBio metagenomics tools:
We are actively developing and adding tools to our metagenomics repo: https://github.com/PacificBiosciences/pb-metagenomics-tools
I always appreciate feedback and proposals for new tools. Please contact me directly at email@example.com to start a discussion.
For additional information, please reach out to me directly at firstname.lastname@example.org, and I can answer your questions or direct you to the best contact at PacBio.